abstract：:The generally accepted concept that the replication of Rous sarcoma virus (RSV) is dependent on host cell DNA synthesis was reexamined. As the host we used terminally differentiated myotubes (MT), in which no cellular DNA synthesis is observed. As an extension of our previous study which indicated that RSV-infected MT...

journal_title：Acta virologica

authors： Park HT,Hara H,Fujihara M,Hsia KJ,Tanaka A,Furuse T,Iwasaki Y,Kaji A

更新日期：1995-09-01 00:00:00

abstract：:The influenza virus M2 protein has an ion channel activity that permits ions to enter the virion during its uncoating and also modulates pH of intracellular compartments. M2 protein is a homotetramer consisting of either a pair of disulfide-linked dimers or a disulfide-linked tetramer. The M2 trans-membrane domain pep...

journal_title：Acta virologica

authors： Ciampor F,Cmarko D,Cmarková J,Závodská E

更新日期：1995-06-01 00:00:00

abstract：:Some differences were established between mopyridone-sensitive (MCU-s) wild strain and mopyridone-resistant (MCU-r) mutant progenies of influenza virus A/Hong Kong/1/68 (H3N2). The virions of MCU-r mutant had a lower buoyant density in linear sucrose gradient as compared to those of MCU-s strain, and an increased abil...

journal_title：Acta virologica

authors： Wassilewa L,Uzunov S,Galabov AS,Simonov AN,Markushin S,Alexandrov M

更新日期：1995-04-01 00:00:00

abstract：:The effect of storage conditions on the serological activity of two strains of potato virus S (PVS), Andean and the ordinary, was studied by ELISA. Virus purificates, infected leaves and their homogenates, stored in lyophilized, frozen and dissolved form at various temperatures were tested. Virus purificates were most...

journal_title：Acta virologica

authors： Cerovská N,Filigarová M

更新日期：1995-02-01 00:00:00

abstract：:Coxiella burnetii cells in both phase I and II reveal in sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-PAGE) similar protein profiles with only small differences. C. burnetii protein profile in SDS-PAGE depended on the method of purification of C. burnetii cells from chick embryo yolk sacs. Immune mou...

journal_title：Acta virologica

authors： Lukácová M,Kazár J,Gajdosová E

更新日期：1994-10-01 00:00:00

abstract：:This study reports on our experience with whole herpes simplex vaccines in Bulgaria for a period of 17 years. More than 1,500 immunized patients with herpes ophtalmicus showed a reduction of the recovery period, reduced number of the recurrences and reduced risk of visual damage. More than 14,000 patients suffering fr...

journal_title：Acta virologica

authors： Dundarov S,Andonov P

更新日期：1994-08-01 00:00:00

abstract：:Mouse polyclonal antibodies were prepared against broad bean stain virus (BBSV, Comovirus group) and its coat protein subunits, large (L) and small (S) protein. These subunits were less immunogenic than native virus. Antibodies against L protein (Anti-L) and native virus (Anti-BBSV) did not react in immunoblots with S...

journal_title：Acta virologica

authors： Subr Z,Matisová J,Gallo J

更新日期：1994-06-01 00:00:00

abstract：:Molecular analysis of histological hallmarks (neurofibrillary tangles, neuritic plaques, neuropil threads and dystrophic neurites) of Alzheimer diseased brain tissues showed that these lesions contain paired helical filaments. Their major constituent is microtubule associated protein tau that is in an abnormally hyper...

journal_title：Acta virologica

authors： Novák M

更新日期：1994-06-01 00:00:00

abstract：:Chromosomal DNAs isolated from eight individuals from the Slovak population and from lymphoblastoid Namalwa cells were analyzed for the presence of genes coding for three subvariants of human interferon-alpha 2 (IFN-alpha 2), namely a, b, and c. The respective genes are regarded allelic, because they differ in the cod...

journal_title：Acta virologica

authors： Kaluz S,Kabát P,Gibadulinová A,Vojtassák J,Fuchsberger N,Kontsek P

更新日期：1994-04-01 00:00:00

abstract：:Induction of porcine interferon (PoIFN) with different lectins (phytohaemagglutinin, Lens culinaris lectin, Arachis hypogea lectin) in various concentrations (500, 50 and 5 micrograms/ml) was studied. Kinetics of PoIFN appearance was analyzed by antiviral and antiproliferative tests in human transformed and nontransfo...

journal_title：Acta virologica

authors： Filipic B,Golob A,Struna T,Likar M

更新日期：1994-04-01 00:00:00

abstract：:Restriction endonuclease fragment length polymorphism (RFLP) analysis and polymerase chain reaction (PCR) were used to characterize the genotypic diversity of 7 strains of Rickettsia conorii from South Africa, Ethiopia, Morocco, India and Russia. The strains of R. conorii were divided into four genotypes by Rsa I or P...

journal_title：Acta virologica

authors： Zhang XF,Fan MY,Chen J,Bi DZ

更新日期：1994-02-01 00:00:00

abstract：:The equine influenza virus, Ludhiana/5/87, isolated from the clinical material during the epidemic of equine influenza in India in 1987 was inhibited in haemagglutination-inhibition test by the antiserum against the prototype A/eq/Prague/1/56 (H7N7) virus and by post-epidemic horse sera. In haemagglutinin and neuramin...

journal_title：Acta virologica

authors： Singh G

更新日期：1994-02-01 00:00:00

abstract：:Eperythrozoon was detected in suckling mice inoculated with a suspension of nymphal Ixodes ricinus ticks. The organism was filterable through 220 nm Millipore membranes, moderately sensitive to diethyl ether, pathogenic to suckling but not to adult mice when given intracerebrally, intraperitoneally or subcutaneously, ...

journal_title：Acta virologica

authors： Hubálek Z,Juricová Z,Halouzka J

更新日期：1993-12-01 00:00:00

abstract：:A strain of rickettsiae, designated Crimea-108, was isolated from ticks Dermacentor marginatus in the Crimea in 1977. Its immunobiological characteristics involve low pathogenicity for experimental animals, moderate infectivity for chick embryos, and antigenic relatedness to spotted fever group (SFG) rickettsiae (R. s...

journal_title：Acta virologica

authors： Balayeva NM,Demkin VV,Rydkina EB,Ignatovich VF,Artemiev MI,Lichoded LYa,Genig VA

更新日期：1993-12-01 00:00:00

abstract：:Six monoclonal antibodies (MoAbs) directed against glycoproteins of bovine leukaemia virus (BLV) were prepared and characterized. Comparison of these MoAbs with anti-gp51 MoAbs of known epitope specificity by competition antibody binding assay allowed to distinguish two new conformational epitopes C1 and C2 on the mol...

journal_title：Acta virologica

authors： Orlík O,Bán J,Gieciová E,Altanerová V,Altaner C

更新日期：1993-10-01 00:00:00

abstract：:The results of the study on the combined antiviral activity of the SHS-174 preparation (a lyophylized infusion from three higher plants) and three amantadine derivatives (rimantadine, amantadine glucuronide and its derivative) are presented. The antiviral effect of the drugs on the reproduction of influenza virus stra...

journal_title：Acta virologica

authors： Serkedjieva J,Zgorniak-Nowosielska I

更新日期：1993-08-01 00:00:00

abstract：:Defective viral particles (DVP) were isolated from the medium of chicken myeloblasts transformed by avian myeloblastosis virus (AMV) in the absence of helper viruses (non-producer myeloblasts). A 60S genomic RNA complex of AMV was isolated from DVP and translated in vitro. The predominant translation product was the g...

journal_title：Acta virologica

authors： Malý A

更新日期：1993-04-01 00:00:00

abstract：:Biological properties of antigens of whole cells of C. burnetii in phase I (WCI), their residue (CMR) and extract (CME) obtained by extraction of C. burnetti with chloroform/methanol mixture were subjected to a comparative study. It was shown that CME is able to react specifically with sera containing antibodies again...

journal_title：Acta virologica

authors： Tokarevich NK,Daiter AB,Polotsky YE,Lukácová M,Kazár J,Kartseva NA,Kuzina VA,Efremov VE,Belov AG

更新日期：1993-02-01 00:00:00

abstract：:In the blood of patients with rheumatoid arthritis and/or sclerodermia systematica usually acid-labile interferon-alpha (IFN-alpha) was found. Blood leukocytes cannot be considered the source of its production as they spontaneously produce IFN-gamma identified with specific antiserum. Blood leukocytes of tested patien...

journal_title：Acta virologica

authors： Scheglovitova ON,Balabanova RM,Kulieva AM,Seilanov LS,Anikina NV,Orlova TG

更新日期：1993-02-01 00:00:00

abstract：:Four isolates of measles virus (Gag, Il, Buk and Shed) were obtained from suspensions of mononuclear cells from patients at the active stage of the disease. Vero cells were used for the virus isolation. All the isolates caused in the infected cell culture the appearance of symplasts of differently sized, star- or spin...

journal_title：Acta virologica

authors： Tikhonova NT,Mamaeva TA,Naumova MA,Leschinskaja EV,Volkov MV,Martynenko IM

更新日期：1992-12-01 00:00:00

abstract：:Invasiveness of Shigella flexneri M90T in HeLa cells was significantly increased when cells were preinfected with poliovirus 1, coxsackievirus B3 and echovirus 6. This effect was dependent on the dose of virus used, evident at early stages of viral infection and lasted hours before the appearance of a cytopathic effec...

journal_title：Acta virologica

authors： Marchetti M,Conte MP,Longhi C,Nicoletti M,Seganti L,Orsi N

更新日期：1992-10-01 00:00:00

abstract：:The efficacy of hepatitis B virus (HBV) vaccine alone (group I) or in combination with hepatitis B immunoglobulin (HBIG) (group II) for prevention of perinatal transmission of the virus was assessed in 21 and 24 neonates, respectively. 58 infants who could not be vaccinated constituted the control group. It was observ...

journal_title：Acta virologica

authors： Sehgal A,Gupta I,Sehgal R,Ganguly NK

更新日期：1992-08-01 00:00:00

abstract：:A herpes simplex virus type 1 (HSV-1)-specific transfer factor (TF), was separated and purified from the leukocyte dialysate of goats immunized with HSV-1 using affinity chromatography on antigen-sorbent and reversed phase high performance liquid chromatography (RP-HPLC). The antigen-specific activities of the startin...

journal_title：Acta virologica

authors： Qi HY,Wan ZF,Su CZ

更新日期：1992-05-01 00:00:00

abstract：:An assay protocol based on exploiting the polymerase chain reaction (PCR) for the direct detection of influenza B virus in throat swabs is described. By the use of PCR with nested primers, it was possible to detect the virus in throat swabs. Dilution experiments showed that as little as 1 plaque forming unit of virus ...

journal_title：Acta virologica

authors： Yamada A,Imanishi J

更新日期：1992-05-01 00:00:00

abstract：:The coat proteins of both ordinary (PVSO) and Andean (PVSA) strains were shown to be similar in molecular weights, peptide maps, amino acid analysis and reaction to a range of carlavirus antiseras. ...

journal_title：Acta virologica

authors： Foster GD,Mills PR

更新日期：1992-03-01 00:00:00

abstract：:Mouse sera collected from day 4 to day 133 postinfection (p.i.) with phase I Coxiella burnetii strain Nine Mile were analysed by immunoblotting with phase I C. burnetii cell lysate. Antibodies of IgG class protein antigens were revealed already on day 10 p.i. (60, 49 and 27 kD proteins), followed by those to 77 kD pro...

journal_title：Acta virologica

authors： Novák M,Brezina R,Kazár J

更新日期：1992-01-01 00:00:00

abstract：:A gene bank of Rickettsia prowazekii strain E constructed in the phage vector lambda EMBL4 was screened for antigen production with anti-R. prowazekii serum. One of the immunoreactive clones, grown at 37 degrees C exhibited the expression of at least two antigens of molecular weight (M(r)) 37 kD and 14 kD. Subcloning ...

journal_title：Acta virologica

authors： Aniskovich LP,Motin VL,Balayeva NM,Smirnov GB

更新日期：1992-01-01 00:00:00

abstract：:Acute isolates of Coxiella burnetii possess a 36-kbp plasmid termed QpH1. DNA hybridizations show that QpH1 contains approximately 6-kbp region of DNA which is not present in the QpRS plasmid from chronic isolates. This QpH1-specific region of DNA contains the contiguous EcoRI fragments G, E, and D. The GED region was...

journal_title：Acta virologica

authors： Minnick MF,Small CL,Frazier ME,Mallavia LP

更新日期：1991-11-01 00:00:00

abstract：:Histology of skin lesions and demonstration in them of Rickettsia conorii by direct immunofluorescence test (DIF) are presented in 13 patients with Mediterranean spotted fever (MSF). The lymphohistiocytic vasculitis which dominated the picture is not specific, however, it could be suggestive for the diagnosis of ricke...

journal_title：Acta virologica

authors： Dujella J,Morović M,Dzelalija B,Gverić M,Novaković S

更新日期：1991-11-01 00:00:00

abstract：:Human choriocarcinoma cells of the JAR line, with no demonstrable surface CD4 receptor were infected with human immunodeficiency virus type 1 (HIV-1), strain RF. Primer-directed enzymatic DNA amplification (polymerase chain reaction, PCR) detected the presence of viral DNA when the cultures were investigated at day 5 ...

journal_title：Acta virologica

authors： Zachar V,Ebbesen P

更新日期：1991-09-01 00:00:00

abstract：:Spleen cells from mice infected with human adenovirus type 6 (Ad6) showed defective interleukin 2 (IL2) production 3-5 days after the infection. The response of spleen cells to exogenous IL 2 was also deficient. The impaired capacity of concanavalin A--(Con A)-activated spleen cells from Ad6--infected mice to utilize ...

journal_title：Acta virologica

authors： Berencsi K,Bakay M,Béládi I

更新日期：1991-08-01 00:00:00

abstract：:A total of 14,250 haematophagous biting midges (genus Culicoides, Diptera: Ceratopogonidae) were collected in June 1986 in the mountains Ceskomoravská vysocina (about 600 m above sea level); from these 2 strains of Tahyna virus (serogroup California, Bunyaviridae) were isolated. To our knowledge this is the first isol...

journal_title：Acta virologica

authors： Halouzka J,Pejcoch M,Hubálek Z,Knoz J

更新日期：1991-05-01 00:00:00

abstract：:A rat glioma cell line, C6-BU-1, showed differential susceptibility to herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2), namely, all the HSV-1 strains tested so far persisted in this cell line but the HSV-2 strains did not. Two clones were derived from C6-BU-1 cells and designated C-17 and C-72. The C-72 as well...

journal_title：Acta virologica

authors： Sakihama K,Eizuru Y,Minamishima Y

更新日期：1991-04-01 00:00:00

abstract：:Human rotavirus strain 649 was isolated from a child with clinical picture of acute intestinal infection in the West European part of the U.S.S.R. This strain was adapted to MA-104 (rhesus monkey kidney) cells. By electrophoresis and haemagglutination it differed from the human rotavirus strain Wa and from the simian ...

journal_title：Acta virologica

authors： Gudkov VG,Rytik PG,Shumko VV,Virinskaya AS,Nepljuev IV,Ilkevich JG

更新日期：1991-01-01 00:00:00

abstract：:A total of 2922 small terrestrial mammals of 12 species were collected in six localities of West Slovakia between 1981 and 1986. When examined for the prevalence of neutralizing antibodies (NA) to Central European encephalitis (CEE) virus we found that 14.6% had antibody. Nearly all (97%) of the 426 animals with antib...

journal_title：Acta virologica

authors： Kozuch O,Labuda M,Lysý J,Weismann P,Krippel E

更新日期：1990-12-01 00:00:00

abstract：:We present serological evidence of infection with western and eastern type of haemorrhagic fever with renal syndrome (HFRS) virus in patient's sera from Slovakia by indirect immunofluorescence. Treatment of sera with 2-mercaptoethanol decreased levels of haemagglutination-inhibition (HI) antibodies to the eastern type...

journal_title：Acta virologica

authors： Gresíková M,Tkachenko EA,Sekeyová M

更新日期：1990-08-01 00:00:00

abstract：:Immobilization stress (hypokinesis) in Balb/c mice may aggravate asymptomatic infection with Langat virus (strain TP-21) as evidenced by 4-fold increased lethality in comparison with control animals. The virus levels in the spleen and brain of stressed and infected mice and the in vitro yield of the virus in immunocom...

journal_title：Acta virologica

authors： Ozherelkov SV,Khozinsky VV,Semenov BF

更新日期：1990-05-01 00:00:00

abstract：:In 1986 five avian paramyxovirus (PMV) strains were isolated in embryonated chicken eggs from sick children with influenza. The strains were identified as PMV-2 serotype due to the close antigenic relationships between their HN-proteins and of the reference PMV-2 strains isolated from different birds all over the worl...

journal_title：Acta virologica

authors： Yakhno MA,Govorkova EA,Kubínova I,Ivanova VT,Stumpa A,Tůmová B

更新日期：1990-04-01 00:00:00

abstract：:Two monoclonal antibodies (MoAb) to the Skalica virus from the tick-borne encephalitis (TBE) complex were used to compare Karshi and Royal Farm viruses with the Russian spring-summer encephalitis, Central European encephalitis (Hypr and Kumlinge strains) Skalica, Langat and Powassan viruses. The first MoAb was prepare...

journal_title：Acta virologica

authors： Gresíková M,Sekeyová M

更新日期：1990-02-01 00:00:00

abstract：:In the years 1980-1984, one paramyxovirus type 4 and 11 influenza viruses were isolated from cloacal swabs collected from migratory waterfowls in Fed. Rep. Germany. One influenza virus of H4N8 subtype was isolated from swabs of commercial ducks collected at an abbatoir. Seven of 10 influenza strains, isolated from mal...

journal_title：Acta virologica

authors： Tůmová B,Bachmann PA,Eichhorn W,Pfleger A,Stumpa A

更新日期：1989-12-01 00:00:00